Resources

Here you can find our in-house protocols for nuclei preparation.

• Nuclei isolation with gradient
  • Protocol for nuclei isolation without fixation: NO_FIXATION_Nuclei_isolation_with_gradient.pdf
  • Protocol for nuclei isolation for subsequent fixation for 10x Genomics Flex or Parse Biosciences: FOR_FIXATION_Nuclei_isolation_with_gradient.pdf

Protocols for nuclei/cell fixation are available on the companies webpages:

• 10x Genomics FLEX
• Parse Biosciences:
  
  • Nuclei fixation v3
  • Cell fixation v3
  • Low input cell and nuclei fixation v3