Microscopes

 

 

Laser scanning confocal microscope Zeiss LSM 800

Description

ZEISS Laser Confocal Scanning Microscope with super-resolution Airyscan. Incubation chamber for live imaging with controlled CO2 and temperature. Microscope is equipped with four solid state lasers, a GaAsP PMT detectors and one T-PMT detector for transmission light detection.

Lasers

405, 488, 561, 640 nm

Detectors

1 x GaAsP PMT, 1 x T- PMT detector

Objectives

Plan-Apochromat 10x / 0.45 AIR, Plan-Apochromat 25x / 0.80 IMM Plan-Apochromat 40x / 1.3 OIL, C-Apochromat 40x / 1.2 W, Plan-Apochromat 63x / 1.4 OIL.

Fluorescence light source

HXP 120V

Filters for fluorescence

DAPI (F-set 49), GFP (F-set 10), Rhodamin (F-set 20),

Transmitted light techniques

Brightfield

Software

ZEN Blue

Location

3.3.14

Reference

 

 

Laser scanning confocal microscope Leica SP8

Description

Inverted microscope Leica SP8 is an automated inverted confocal laser scanning microscope with a very flexible white light laser source and super-sensitive Hybrid detectors (HyD).

Lasers
  • Diode Lasers: 405 nm
  • Argon Laser: 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
  • White light laser (WLL): continuous laser output in the range of 470-670nm with AOTF

Detectors

2 x super-sensitive Hybrid detectors (HyD), 1 x T- PMT detector

Objectives

HC Plan-Apochromat 10x / 0.40 AIR, HC Plan-Apochromat 20x / 0.75 IMM, HC Plan-Apochromat 40x / 1.1 W, HC Plan-Apochromat 63x / 1.40 OIL.

Fluorescence light source

HXP 120V

Filters for fluorescence

Name

Excitation

Dichroic

Emission

DAPI

BP340-380

400

LP425

GFP

BP450-490

510

LP515

DsRed

BP515-560

580

LP590

Transmitted light techniques

Brightfield

Software

LASX

Location

4.3.26

Reference

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Delta Vision Elite MicroscopeDescription 

Inverted decovolution fluorescence widefield microscope with XYZ motorized stage/focus control, environmental control for live cell imaging, time lapse, multi point, and tile scanning from Olympus.

Fluorescence light source High power LED illumination system: Lumencor SpectraX light engine (https://lumencor.com/products/spectra-x-light-engine/)
Objectives UPlanSApo 10x/ 0.4 AIR, LCPlanFl 20x / 0.4 AIR, UApo/340 40x / 1.35 Oil, PlanApoN 60x/ 1.42 OIL, UPlanSApo 100x/ 1.4 OIL.
Filters for fluorescence
Name Excitation Dichroic Emission
DAPI BP 395/25 410 BP 435/26
FITC BP 470/24 490 BP 515/30
TRITC BP 550/15 570 BP 595/40
CY5 BP 640/30 660 BP 705/72
CFP BP 483/32
YFP BP 544/23
Transmitted light techniques Brightfield and DIC
Camera Photometrics CoolSNAP HQ camera
Software SoftWoRx V 7.2.2
Format 4.3.12
Reference https://www.imsol.co.uk/deltavision-elite/

 

 

EVOS microscope

Description

Fully-automated, inverted, multi-channel fluorescence and transmitted light imaging system coupled with capabilities for multi-position well scanning, Z-stack, tile-stitching, live-cell imaging, time lapse, and autofocus from Thermofisher Scientific.

Fluorescence light source LED light sources
Objectives 4X (AMEP4680), 10X (AMEP4681), 20X (AMEP4982), 40X (AMEP4683 and  AMEP4754)
Filters for fluorescence

GFP        Ex 482/25,  Em 524/24

RFP         Ex 542/20,  Em 593/40
Transmitted light techniques Brightfield
Camera Colour and Monochrome camera
Software Auto 2
Format Wellplate and slides
Location 3.4.10
Reference https://www.thermofisher.com/dk/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems.html